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KMID : 0921620140440020152
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Journal of Bacteriology and Virology
2014 Volume.44 No. 2 p.152 ~ p.161
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Aspergillus oryzae S-03 Produces Gingipain Inhibitors as a Virulence Factor for Porphyromonas gingivalis
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DANSHIITSOODOL Narandalai
YAMASHITA Hideyuki
NODA Masafumi
KUMAGAI Takanori
MATOBA Yasuyuki
SUGIYAMA Masanori
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Abstract
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Oral infection with Porphyromonas (P.) gingivalis causes periodontitis that is manifested by the destruction of gingival connective tissues. Although a few types of antibiotics are effective against the infection, its use induces the appearance of drug-resistant bacteria. The present study shows that the fermented product of Aspergillus (A.) oryzae S-03, cultivated on the fat-removed soybean, inhibits the cell growth of the P. gingivalis. Likewise, the fermented product of the S-03 strain cultured for 26~42 h displays an inhibitory activity to gingipain as a virulence factor of P. gingivalis. The activity is not lost even with heat treatment at 100℃ for 15 min. We also demonstrate that the S-03 strain exhibits high protease activity. In addition, the strain does not produce aflatoxin because of the loss of a regulatory gene, aflR, necessary for the toxin biosynthesis.
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KEYWORD
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Apergillus oryzae, Gingipain, Inhibitor, Koji
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